- Title
- Chlorogenic acid potentiates the anti-inflammatory activity of curcumin in LPS-stimulated THP-1 cells
- Creator
- Bisht, Akshay; Dickens, Martin; Rutherfurd-Markwick, Kay; Thota, Rohith; Mutukumira, Anthony N.; Singh, Harjinder
- Relation
- Nutrients Vol. 12, Issue 9, no. 2706
- Publisher Link
- http://dx.doi.org/10.3390/nu12092706
- Publisher
- MDPI AG
- Resource Type
- journal article
- Date
- 2020
- Description
- The anti-inflammatory effects of curcumin are well documented. However, the bioavailability of curcumin is a major barrier to its biological efficacy. Low-dose combination of complimentary bioactives appears to be an attractive strategy for limiting barriers to efficacy of bioactive compounds. In this study, the anti-inflammatory potential of curcumin in combination with chlorogenic acid (CGA), was investigated using human THP-1 macrophages stimulated with lipopolysaccharide (LPS). Curcumin alone suppressed TNF-a production in a dose-dependent manner with a decrease in cell viability at higher doses. Although treatment with CGA alone had no effect on TNF-a production, it however enhanced cell viability and co-administration with curcumin at a 1:1 ratio caused a synergistic reduction in TNF-a production with no impact on cell viability. Furthermore, an qRT-PCR analysis of NF-KB pathway components and inflammatory biomarkers indicated that CGA alone was not effective in reducing the mRNA expression of any of the tested inflammatory marker genes, except TLR-4. However, co-administration of CGA with curcumin, potentiated the anti-inflammatory effects of curcumin. Curcumin and CGA together reduced the mRNA expression of pro-inflammatory cytokines [TNF-a (~88%) and IL-6 (~99%)], and COX-2 (~92%), possibly by suppression of NF-KB (~78%), IKB-β-kinase (~60%) and TLR-4 receptor (~72%) at the mRNA level. Overall, co-administration with CGA improved the inflammation-lowering effects of curcumin in THP-1 cells.
- Subject
- curcumin; chlorogenic acid; bioactive combination; inflammation; NF-KB pathway
- Identifier
- http://hdl.handle.net/1959.13/1428080
- Identifier
- uon:38604
- Identifier
- ISSN:2072-6643
- Rights
- © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
- Language
- eng
- Full Text
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